Zenbiohumanpre-ADIpocytes(adiposederivedadultstemcells)andplacentalmesenchymalstemcells(MSC)arecharacterizedbytheirself-renewingcapacityandABIlitytodifferentiateintochondrocytes,adipocytes,andosteocytes.Thismakesthemattractivestartingmaterialsfortissueengineeringandregenerativemedicineapplications.

Sincefewtransplantedcellspersistinvivo,thebeneficialeffectsofcelltherapymaylieinthesecretedfactorsbeingtheactivecomponentofthistreatment.AkeypartofparacrinesecretionisExosomes,whicharemembranevesiclesthatarestoredintracellularlyinendosomalcompartmentsandaresecretedwhenthesestructuresfusewiththecellplasmamembrane.

• Exosomescontainprotein,DNA,andRNA,thusmakingthemanattractivevectorofparacrinesignalsdeliveredbystemcells.
• Exosomesmayalsobe"loaded"withpredeterminedproteinsandnucleicacidtoachieveadesiredeffect(1).
• Exosomescanbestoredasan"off-the-shelf"producthavingthepotentialforcircumventingmanyofthelimitationsofviablecellsfortherapeuticapplicationsinregenerativemedicine.
• Invitro,exosomesfrompre-adipocytesstimulatecellproliferationinawoundhealingmodel.
• Invivo,adipose-graftderivedexosomeshavebeenshowntobeapromisingtoolforskinrepairandremodeling(2)

QualityControl:AllcellshavebeenscreenednegativeforHIV-1,HIV-2,Hep-B,Hep-CandSyphilis.AllisolatedandconcentratedexosomesundergoincludesParticlemeandiameter,Proteinconcentration,RNAconcentrationandConcentrationofparticles/ml.

Exosome-mediatedfibroblastproliferation

Afterthecellmonolayerwas"wounded"usingacellscraper,thecultureswerewashedandfedwithDMEM+10%exosome-freeFBS(Control),orDMEM+10%exosome-freeFBSsupplementedwith25µgofpre-adipocyteexosomes(ExosomeTreated).Aftera5dayincubation,fibroblastproliferationclosedthegapbetweenthetwoedgesofthewoundedmonolayeronlyinthemediumsupplementedwithexosomes.Thesedatasupportthenotionthatfactorsincludingexosomessecretedbypre-adipocytes(adiposetissue-derivedMSC)playaroleinwoundhealingandtissueregeneration,byfacilitatingcellularproliferation

Zenbio成立于1995年，自其成立起就在持续供货和扩大研发方面一直处于领先地位，Zenbio以其国际水平的技术支持和专注的客户服务，得到了广泛的认可。Zenbio的成立致力于满足研究人员对肥胖者、糖尿病和心血管疾病研究的迫切需要。尽管啮齿类动物模型对研究新陈代谢各种通路极为有用，但人体的病理学研究关键分析仍需要适合的体外模型，用以研究人的脂肪细胞。而Zenbio能够为研究者提供多种细胞作为病理学研究模型。产品主要包括人类原代细胞、细胞系、培养基、及代谢疾病和药用化妆品等方面的检测试剂盒。