FreerADIcalsandreactiveoxygenspecies(ROS)arehighlyreactivemoleculesthataregeneratedbynormalcellularprocesses,environmentalstresses,andUVirradiation.ROSreactwithcellularcomponents,damagingDNA,carbohydrates,proteins,andlipidscausingcellularandtissueinjury.Excessproductionofreactiveoxygenspeciescanalsoleadtoinflammation,prematureagingdisorders,andseveraldiseasestates,includingcancer,diabetes,andatherosclerosis.Organismshavedevelopedcomplexantioxidantsystemstoprotectthemselvesfromoxidativestress,however,excessROScanoverwhelmthesystemsandcauseseveredamage.

TheZenbioORAC(OxygenRadicalAbsorbanceCapacity)AntioxidantAssayKitcanbeusedtodeterminethetotalantioxidantcapacityofBIOLOGicalfluids,cells,andtissue.Itcanalsobeusedtoassaytheantioxidantactivityofnaturallyoccurringorsyntheticcompoundsforuseasdietarysupplements,topicalprotection,andtherapeutics.Theassaymeasuresthelossoffluoresceinfluorescenceovertimeduetoperoxyl-radicalformationbythebreakdownofAAPH(2,2"-azobis-2-methyl-propanimidamide,dihydrochloride).Trolox[6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylicacid],awatersolublevitaminEanalog,servesasapositivecontrolinhibitingfluoresceindecayinadosedependentmanner.TheORACassayisakineticassaymeasuringfluroesceindecayandantioxidantprotectionovertime.Theantioxidantactivityinbiologicalfluids,cells,tissues,andnaturalextractscanbenormalizedtoequivalentTroloxunitstoquantifythecompositeantioxidantactivitypresent.ThisassaymeasuresantioxidantactivitybyhydrogenatomtransferandwhencombinedwithZenBio"sABTSantioxidantassaykit,providesacomprehensiveanalysisofatestsample"santioxidantactivity.

Zenbio成立于1995年，自其成立起就在持续供货和扩大研发方面一直处于领先地位，Zenbio以其国际水平的技术支持和专注的客户服务，得到了广泛的认可。Zenbio的成立致力于满足研究人员对肥胖者、糖尿病和心血管疾病研究的迫切需要。尽管啮齿类动物模型对研究新陈代谢各种通路极为有用，但人体的病理学研究关键分析仍需要适合的体外模型，用以研究人的脂肪细胞。而Zenbio能够为研究者提供多种细胞作为病理学研究模型。产品主要包括人类原代细胞、细胞系、培养基、及代谢疾病和药用化妆品等方面的检测试剂盒。